Catalytic roles of βLys87 in tryptophan synthase: 15N solid state NMR studies
نویسندگان
چکیده
منابع مشابه
Accurate measurement of 13C-15N distances with solid-state NMR.
Solid-state NMR technique for measuring distances between heteronuclei in static powder samples is described. It is based on a two-dimensional single-echo scheme enhanced with adiabatic cross polarization. As an example, the results for intramolecular distances in alpha-crystalline form of glycine are presented. The measured NMR distances (13)C(alpha)-(15)N and (13)C(')-(15)N are 1.496+/-0.002 ...
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The bulky and amphiphilic nature of tryptophan residues makes them particularly interesting components of proteins. In bacteriorhodopsin, four of the eight tryptophan residues are in the active site, forming parts of the retinal binding pocket. In this work, we use solid-state NMR to study the interactions of the tryptophan residues in wild-type bacteriorhodopsin, in the resting state, and in c...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
سال: 2015
ISSN: 1570-9639
DOI: 10.1016/j.bbapap.2015.02.003